In this application note we highlight the capability of Sony’s CGX10 cell isolation system using microfluidics based closed cell sorting technology to isolate target cell populations used in cell therapy protocols. The results demonstrate that the CGX10 can isolate cell fractions present in low and abundant levels at high throughput. The results also indicate that the CGX10 Cell Isolation System is able to deliver highly pure and viable cell subsets and outperforms a traditional cuvette sorter.