In this study, our considerations were to determine the best fluorochrome set to use for 40- to 45-color panels on the ID7000. We aimed to incorporate a set of core markers that utilize the most challenging fluorochrome combinations while leaving the less challenging fluorochromes available for substitutions by users to make derivative panels. We used tools such as the Spectral Reference Library and the Spillover Spreading Matrix to test for ideal fluorochrome combinations, with at least three more dyes ready to expand the panel size with this five-laser instrument configuration. We also optimized our panel design and gating methods to enable good marker/fluorochrome choices in the future.
