SCREENING OF BASIC IMMUNOLOGICAL MARKERS

The starting point for precise leukocyte phenotyping is identifying the frequency of basic immune cell subpopulations. The prepared panel is a classical yet slightly extended TBNK test, allowing simultaneous analysis of major T cell groups (CD3+, CD4+, CD8+), B cells (CD19+), and NK cells (CD16+/CD56+). The addition of markers such as CD14 enables the evaluation of monocyte presence and function, while CD45 allows for an accurate determination of the total leukocyte count. The extended panel provides a broad range of information on the composition and proportions of various immune cell subpopulations.
This analysis allows for the rapid and precise identification of immune system dysfunctions, which is particularly useful in diagnosing autoimmune diseases, hematological malignancies (e.g., leukemias, lymphomas), and monitoring blood changes in patients undergoing immunosuppressive or immunostimulatory therapies. It is also a key tool in monitoring the course of viral infections, such as HIV, where changes in CD4+ and CD8+ lymphocyte subpopulations can serve as indicators of disease progression.
The ability to simultaneously analyze multiple markers in a single test provides a comprehensive assessment of immune system function, which is invaluable in diagnostics and therapy management. The proposed TBNK+ panel works excellently on a Sony MA900 sorter or a Thermo Fisher Attune NXT flow cytometer. It is an essential tool for anyone seeking a comprehensive overview of a patient’s immunological status, based on modern multi-color flow cytometry technology, which guarantees fast and reliable results. If needed, the panel can be customized to optimize the use of the equipment at your disposal.
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THE STORY OF „T”

The panel is perfectly suited for screening the activation status and maturity of T cells.

1. Naive T cells (CD3+ CD4+ or CD8+, CD45RA+, CCR7+) – Cells that have not yet encountered an antigen.

2. Central memory T cells (TCM, CD3+ CD4+ or CD8+, CD45RO+, CCR7+) – Memory cells that migrate to lymph nodes and are capable of providing a long-term response to antigens.

3. Effector memory T cells (TEM, CD3+ CD4+ or CD8+, CD45RO+, CCR7-) – Effector memory cells found in tissues that can immediately respond to the reappearance of an antigen.

4. Terminally differentiated effector T cells (TEMRA, CD3+ CD8+, CD45RA+, CCR7-) – Highly cytotoxic CD8+ T cells that have completed differentiation.

5. Senescent T cells (CD57+, CD28-) – T cells that have lost their ability to proliferate and often show characteristics of aging.

6. Exhausted T cells (PD-1+) – T cells that have been chronically activated and may be functionally impaired, often seen in chronic infections or cancers.

The panel is designed for the 3-laser Sony MA900 sorter. Contact us, and we’ll customize the panel for your equipment.

Treg Research Panel

The Treg Check panel can be used for detailed analysis of regulatory T cell (Treg) populations and their activation and function. This panel is particularly useful in studies on immunoregulation, autoimmune diseases, cancers, and immunomodulatory therapies.

1. Identification and Characterization of Tregs:

   The key objective of this panel is to identify Tregs (CD4+, CD25high, FoxP3+), which are responsible for suppressing excessive immune responses and maintaining immune tolerance.
   Adding markers such as Helios and CD39 allows for a more precise distinction between natural Tregs and those induced by inflammation or therapy.

2. Evaluation of T Cell Activation and Function:

   CD25 and CD39 indicate Treg activation levels and their potential to suppress immune responses.

3. Research on Autoimmune Diseases and Inflammatory Conditions:

   This panel can be used to monitor changes in Treg populations and their functions in diseases like rheumatoid arthritis, multiple sclerosis, type 1 diabetes, or systemic lupus erythematosus.

4. Cancer Immunotherapy:

   Analyzing Treg populations is crucial in the context of cancers, where excessive Treg activity can suppress the anti-tumor response. Monitoring these cells during immunotherapies can help assess treatment effectiveness.

The panel is designed for the 4-laser ThermoFisher Attune NXT acoustic cytometer. Contact us, and we’ll customize the panel for your equipment.

Michał Maj
mmaj@cyto-logic.pl
+48 782 882 700