Omnicyt

The Omnicyt software enables powerful user-defined experimental analysis via an intuitive, easy-to-use interface. Advanced tools provide simple acquisition and analysis for users at all experience levels.

Ease of use

Intuitive functionality-adjust flow rates, compensation and voltages through intuitive user interface
Less learning time-simple user interface with guided workflows
Easy instrument operation-automated maintenance and instrument performance monitoring

Speed

Quickly run flow cytometry experiments; less scrolling and clicking
Increase productivity; live-streaming update of statistics during acquisition of events
Fast refresh rates for large data sets of up to 20,000,000 events per sample and indeed, it’s posible to add more events with Append option

Guided functionality

Guided functions for automated or easy user-defined compensation
Automated maintenance: Start Up, Shutdown, Rinse, Santize Omnicyt™ SIP, Deep Clean, Sanitize, System Decontamination, Autosampler Calibration
Automated Data and Database backup to ensure data redundancy

Visual management buttons and icons help navigate workflows and running experiments. The software allows for:

Customizable experimental workflows–pre-define settings for plots, gates, experiments, reports, and overlays
Preview screens–easy set-up by showing all plot combinations instantaneously for easy experimental set-up
Guided functions–for automated or easy user-defined compensation

Versatile

  • Heat map for easy setup of plate based assays
  • Sample Recovery to return unused sample to save precious samples
  • Hierarchy view of plots to instantly view complex gating strategies

Customizable

  • User customizable Wait-to-Record function
  • Ability to set user options for default settings for gates, plots, fonts, colors, and group/sample names
  • Visual appearance of the plots is completely customizable; fonts, colors, titles legends and much more can appear exactly the way you want

Publication quality data

  • Smartgate labeling to customize quad gate names and target names
  • Add text, statistics and even images to immediately present data
  • One-click saving of high resolution plots in a variety of file formats including XML, FCS, and even ZIP, for multiple files concurrently
  • Overlay module to perform comparative analysis of single and dual parameter data
  • Preview plots to instantly view all combinations of parameters in a file

User management

  • Levey-Jennings and Performance History reports of baseline and performance tests to monitor trends
  • Ability to create and manage multiple user accounts
  • System access based on user account privileges
    System logs amount of time between user logon / logoff and number of tubes run per account per period of time

Note: The Omnicyt Flow Cytometer includes a dongle and 1 license for the Omnicyt Software plus a dongle and 1 license for the Infinicyt Software The Omnicyt software may be downloaded onto more than one computer, however the software will only run on computers that also have a dongle inserted. Users then can export FCS files in 3.0 and 3.1 format for use in other analysis programs such as Infinicyt Software.

Software simplifies data acquisition with a dashboard that provides the tools for immediately viewing acquisition status and for setting acquisition stopping criteria. The application settings feature automatically adjusts voltage settings to daily changes in cytometer performance, so that users can run existing applications more simply and quickly.

Compensation is both rapid and accurate using a guided software system. Users are only required to prepare the proper controls and adjust the voltage gates. The system adjusts the compensation and applies it to the samples.
Compensation tools

  • Automated compensation setup allows rapid and accurate compensation and helps to eliminate tedious trial and error adjustments
  • Both negative and unstained gating parameters are available
  • On-plot compensation for fine-tune adjustment
  • Modification of compensation to add or remove parameters as needed after compensation is set up
  • Setup and collect compensation controls directly from a plate

(1) Negative gating is useful for heterogeneous samples that are often found in tumor and blood samples. (2) Choose fluorophore-labeled antibodies based on the emitted emission spectrum. Dyes should have minimally overlapping spectra. (3) Prepare single-stained controls. An unstained control is not required. (4) Fluorescent compensation beads can also be used to set compensation parameters when there is not enough sample. (5) Use the guided system and select for negative gate. (6) Run sample and adjust the voltages to see the fluorescent label on the plot. Press Apply Compensation.

(1) Unstained compensation is useful for homogeneous samples such as cell lines or already purified cell populations. (2) Chose fluorophore-labeled antibodies based on the emitted emission spectrum. Dyes should have minimally overlapping spectra. (3) Prepare both unstained and single stained controls. (4) Fluorescent compensation beads can also be used to set compensation parameters when there is not enough sample. (5) Use the guided system and select for unstained gate. (6) Run sample and adjust the voltages to see the fluorescent label on the plot. Press Apply Compensation.