1. Optics:

• 3 lasers
  • Blue laser: 488 nm, 50 mW
  • Violet laser: 405 nm, 50 mW
  • Red laser: 637 nm, 100 mW
  • A fourth yellow laser (561 nm, 50 mW) enabling 16 parameters is available as an upgrade.
• 13 individual detectors:
  • 11 fluorescent detectors
  • 2 of light dispersion (FSC + SSC)
  • Fluorescence sensitivity:
  • <80 MESF for FITC
  • <30 MESF for PE
  • <70 MESF for APC
• Set of optimized filters for clinical cytometry most used fluorochromes:
  • VL-1: 440/50 (PacB; BV421; SB436; PR436)
  • VL-2: 512/25 (OC515; PacO*; AmCyan; BV510)
  • VL-3: 603/48 (BV605; PacO*; SB600; PR600)
  • VL-4: 710/50 (BV711; SB702)
  • BL-1: 530/30 (FITC)
  • BL-2: 574/26 (PE)
  • BL-3: 695/40 (PerCP-Cy5.5)
  • BL-4: 780/60 (PE-Cy7)
  • RL-1: 670/14 (APC)
  • RL-2: 720/30 (Alexa700; APC-A700)
  • RL-3: 780/60 (APC-C750; APC-H7; APC-Cy7)

*This fluorochrome can be detected by several filters.

• Flat-top parallel lasers with fixed alignment:
  • High stability and sensitivity
  • Lower calibration frequency

2. Fluidics:

Volumetric system:

• • Allows absolute cell counts (cells/µL)
  • Low fluid consumption: no fluidics cart is required
  • Allows different types of tubes (Ø 17×100 mm and 8.5×45 mm)
  • Recovery of unacquired volumes in loaded samples

Acoustic assisted hydrodynamic focusing:

• • Higher reproducibility and accuracy even at high rates
  • Flow rate between 12.5 and 1,000 µL/min: 12.5, 25, 100, 200, 500 and 1.000µL/min
  • Higher acquisition rate of diluted samples
  • The acoustic focusing system works only from the flow rate: 100µL/min. At 12.5 and 25µL/min, the fluidic system works as a standard hydrodinamic system

Anti-clogging technology:

• • Reduced risk of clogging even with high concentration samples

3. Performance:

Digital system with 20 bits and 1,048,576 channels.

• • Electronic pulse: area, height and width for all detectors.
  • Acquisition rate: up to 35,000 events/sec*.

*This is the electronic resolution at optimal conditions; diagnostic samples are not intended to be acquired at this rate.
4. Acquisition software:

Full matrix compensation, in manual or automatic modes.

• • Up to ~20 million events can be recorded in a single file.
  • Prevents data loss since aborted events, including aggregates, are recorded. Notice that aggregates may contain cells of interest such as plasma cells*.

* Files may present higher percentage of doublets than expected.

• • Running stops when acquisition problems, such as bubbles, are detected.

5. Quality

Manufacturing standards comply with ISO 13485:2016
CE-IVD

6. Cytometer dimensions
Benchtop cytometer with fluid bottles stored within:

• • 40 x 58 x 43 cm (H x W x D)
  • Weight: 29 Kg

No fluidics cart is required.

7. Consumables

• • Performance Tracking Beads (3mL) (CYT-OMNI-PTB)
  • Focusing Fluid (10L) (CYT-OMNI-FF)
  • Debbuble Solution (50mL) (CYT-OMNI-DBS)
  • Shutdown Solution (250mL) (CYT-OMNI-SD)
  • Washing Solution (250mL) (CYT-OMNI-WS)