REAGENTS FOR THE MAINTENANCE OF CYTOMETERS AND CELL SORTERS

Proper maintenance of flow cytometers and cell sorters primarily requires regular rinsing and/or cleaning of the instrument’s fluidics and pressure systems. The most critical aspect of these maintenance activities is preventing the formation of microbial biofilms in the capillaries and tubing, as well as the precipitation of salts from the sheath fluid.

Equally important is the implementation of quality control (QC) practices, which are part of good cytometry practices. QC in flow cytometry is essential to ensure accurate, reliable, and reproducible data from experiments. It encompasses a series of practices, protocols, and controls designed to verify that the flow cytometer operates optimally and that experimental results are consistent over time and across different devices. QC includes:

• Calibration and standardization of instruments,

• Monitoring instrument functions (sensitivity, stability, and compensation settings over time),

• Fluorescence compensation,

• Quality control of antibodies and reagents,

• Standardization of data acquisition protocols,

• Data analysis monitoring (result reproducibility and the creation of standard operating procedures (SOP) for each application),

• Monitoring correct instrument usage and troubleshooting.

Cyto-Logic highly recommends following products from our partners portfolio:

• DPBS, no calcium, no magnesium (Thermo Fisher Scientific) – Cat. No. 14190144

  A balanced PBS solution without calcium and magnesium ions to limit cell aggregation and salt precipitation in the instrument tubing and capillaries (especially in areas related to optics).

• DPBS (10X), no calcium, no magnesium (Thermo Fisher Scientific) – Cat. No. 14190144

  A 10-fold concentrated balanced PBS solution without calcium and magnesium ions.

• BrightComp eBeads™ Compensation Bead Kits (Thermo Fisher Scientific) – Cat. No. A10514

  A solution containing calibration beads that provide consistent, accurate, and easy-to-use compensation settings in flow cytometry.

• 10X ClearSort™ Sheath Fluid, Sterile, 4 x 1L (SONY Biotechnology) – Cat. No. AE700713

  A balanced PBS solution without sodium azide.

• Automatic Setup Beads Kit (SONY Biotechnology) – Cat. No. LE-B3001

  A solution containing calibration beads for analyzing and sorting parameters in SONY Biotechnology instruments.

• AccuCheck Counting Beads (Thermo Fisher Scientific) – Cat. No. PCB100

  An effective single-platform method for absolute cell counting, combining the benefits of direct flow cytometric immunophenotyping with the use of two different sub-populations of fluorescent beads. Provides a dual internal standard for blood cell concentration measurement.

• 123count eBeads™ Counting Beads (Thermo Fisher Scientific) – Cat. No. 01-1234-42

  Designed for absolute counting of cells or other particles via flow cytometry, enabling precise cell counting and concentration measurement.

• UltraComp eBeads™ Compensation Beads (Thermo Fisher Scientific) – Cat. No. 01-2222-42

  Bind antibodies independently of the immunoglobulin light chain. Designed for compensation using all fluorochromes excited by ultraviolet (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers.

• OneComp eBeads™ Compensation Beads (Thermo Fisher Scientific) – Cat. No. 01-1111-42

  Bind antibodies independently of the immunoglobulin light chain. Can be stained with single fluorochrome-conjugated antibodies, serving as single-color compensation controls.

• 8 Peak Beads (SONY Biotechnology) – Cat. No. AE700522

  Allow for testing the sensitivity and resolution of fluorescence measurement results.

• Sodium hypochlorite, 10-15% active chlorine, Thermo Scientific Chemicals (Thermo Fisher Scientific) – Cat. No. 219250100

  A concentrated sodium hypochlorite solution for sterilizing sample lines in flow cytometers. The recommended final concentration range is 0.7–2%.

• Attune™ Wash Solution (Thermo Fisher Scientific) – Cat. No. J24974

  An optimized wash buffer used to maintain sample line cleanliness, minimizing contamination (chemical, microbial, and cross-sample).

• Attune™ Debubble Solution (1X) (Thermo Fisher Scientific) – Cat. No. A10496

  A solution for removing air bubbles from the pressure-fluidic system of cytometers.

prof. dr hab. Wojciech Juzwa
aplikacje@cyto-logic.pl
+48 889 735 598