Using spectral unmixing, the SA3800 allows maximum flexibility for fluorochrome selection. Results are comparable to those obtained using conventional systems.
10-color Phenotyping of Human TBNK and Mature Erythrocytes using 3 Lasers
In this panel, all lymphocytes were identified by staining with CD45. From the CD45+ population, B cells (CD19), NK cells (CD56), and T cells (CD3) were identified. In addition, mature erythrocytes were identified using CD235. The T-cell population was further analyzed to find effector T cells (CD8) and helper T cells (CD4). These T-cell populations were analyzed to find subset populations, Naïve CD4 and CD8 T cells, and Activated CD8 T cells using HLA-DR and CD45RA.