1985-001-02-Sony_Logo_0002_Warstwa-1

SH800S Cell Sorter

A. Fluorescence sensitivity MESF values measured using Spherotech® 8 peak-beads are: FITC 120 and PE 110. B. Fluorescence resolution was measured using propidium iodide (PI) stained chicken erythrocyte nuclei (CEN). A coefficient of variation of <2.5% was observed for the G0/G1 peak.
Distinct resolution of multicolor samples is seen with a four laser SH800S system. A. Normal human blood was stained with CD3 Alexa Fluor® 488, CD4-PECy7, CD8 Alexa Fluor® 700, CD19 APC, CD16+CD56, PE and HLA-DR BV421. All plots were gated on lymphocytes. B. High-purity sorting of the regulatory T cells (CD3+ CD4+ CD25 high CD127-) population.
Human cell lines co-expressing GFP, YFP, dsRed and CFP using the fluorescent protein optical filters are shown. Refer to the filter guides for the fluorescent protein filter sets.
These plots show a sorting and viability analysis of differentiating mouse embryonic stem cells expressing Isl1Cre-tdTomato. Analysis of the tdTomato (+) purified population is shown. the inset image (E) shows cells in culture 24 hours post sorting.
High-speed sorting of E.coli using a 70-µm sorting sample of cells was sorted at a threshold event rate of 30,000 events/sec at a sort setting of 50 kHz and 40 psi. Post-sort analysis shows that >95% purity was obtained.